Molecular diagnostic tests are progressively moving towards lyophilisation. There are several advantages for this, including room temperature shipping and storage, extended shelf-life, and increased flexibility in sample volume. In order to be compatible with drying, however, enzyme preparations must be glycerol-free and include specialised excipients that preserve the mixture as it is exposed to freezing, vacuum, and dehydration. An ideal lyophilisation formulation, for example, should stabilise an enzyme in a freeze-dried format and allow very fast rehydration and reactivation of the enzyme preparations without impacting its performance post rehydration. Can also be used wet and/or with purified nucleic acid.
Lyo-Ready qPCR master mix is a glycerol-free formulation that contains Taq polymerase, reaction buffer, dNTP, MgCl2, and lyo-excipients. It is ideal for developing room temperature, lyophilised, multiplex qPCR diagnostic tests and is suited for high-throughput, automated platforms requiring milli-second rehydration times.
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