Bst DNA Polymerase is an enzyme derived from the large fragment of Bacillus stearothermophilus DNA Polymerase I. It contains 5´- 3´ DNA polymerase activity and strong strand displacement activity but lacks 5´- 3´ exonuclease activity. The strong strand displacement activity enables Bst DNA Polymerase to synthesiSe DNA at a constant temperature making it an ideal enzyme for isothermal amplification, including HDA, MCA, and Loop-Mediated Isothermal DNA Amplification (LAMP).
Bst DNA Polymerase (8 U/µL) is supplied with an Enzyme Dilution Buffer and a Bst Reaction Buffer formulated to deliver the fastest amplification speed (time-to-results), yield, salt tolerance, and sensitivity, speeding up your product development time and allowing you to commercialise your new products faster. For the most difficult templates, an Inhibitor-Tolerant Bst Buffer is available that is specifically designed for high performance when using crude samples such as sputum or saliva.
- Optimised for isothermal amplification such as LAMP
- Designed for fast polymerization and so faster time to results
- Supplied with a reaction buffer and enzyme dilution buffer
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