MI Broth detects the presence of coliform bacteria by the production of b-galactosidase, which cleaves the substrate MUGal to produce 4-methylumbelliferone, which fluoresces on exposure to UV light. Non-coliforms do not produce this enzyme and therefore do not fluoresce on the medium. Escherichia coli is detected by the compound IBDG. The b-glucuronidase produced by Escherichia coli cleaves the substrate to produce a blue indigo colour in the colonies. As Escherichia coli is also a total coliform, and also produces b-galactosidase, it will also fluoresce. The antibiotic Cefsulodin is present to inhibit the growth of gram-positive bacteria and some non-coliform gram-negative bacteria that can cause false positive reactions.
GVS provides an extensive range of culture broths and solutions for the cultivation, detection and enumeration of bacteria yeast, fungi, viruses, pathogens and moulds. Each nutrient rich liquid media is specifically developed for use in the analysis of drinking water, surface water, milk, juice, beverages, sugar based drinks, food and pharmaceutical samples. All liquid medias undergo detailed quality control checks in accordance with standard methods, guaranteeing uniform preparation every time. Comprehensive end product testing ensures a stable sterile liquid media for optimal culture growth.
- Capacity: 2mL
- Individual pre-portioned vials
This product has met the following criteria to qualify for the following awards: