PP0051 Bacillus cereus Selective Agar (PEMBA) is a differential selective medium for the isolation and enumeration of Bacillus cereus in clinical specimens and food samples. It is particularly suitable for the detection of small numbers of Bacillus cereus particularly in the presence of other contaminating organisms. The medium is also sufficiently diagnostic that colonies of B. cereus are readily identified and confirmed by microscopic examination.
PP0051 Bacillus cereus Selective Agar (PEMBA) is recommended by Public Health England and complies with ISO 11133:2014 and ISO 21871:2006.
The medium is made selective by the inclusion of Polymixin and Sodium Pyruvate is also present which is said to improve Egg Yolk precipitation and enhance sporulation. As Bacillus cereus is Mannitol Negative the colonies are bluish in colour, due to the presence of the Bromothymol Blue Indicator, with a surrounding precipitate of the same colour due to Lecithinase production (from the Egg Yolk).
NB: It should be noted that some Proteus spp. and gram positive cocci may grow on this medium.
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