Heparinase I and III Blend from Flavobacterium heparinum,

Not available outside of the UK & Ireland.

Application

Heparinase I and III Blend from Flavobacterium heparinum has been used in:the digestion of heparan sulfate from ovine vitreous human embryonic kidney cells glycosaminoglycans from arterial tissuesP0 retinaedigestion

Biochem/physiol Actions

Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate.

Heparinase I and III plays vital role in various biological processes: modulate cell-growth factor interactions, cell-lipoprotein interactions, neovascularization. It cleaves highly sulphated polysaccharide chains in presence of 2-O-sulfated α-L-idopyranosyluronic acid and β-D-glucopyranosyluronic acid residues of polysaccharides.

General description

Heparinase is an inducible, non-extracellular heparin-degrading enzyme. Three types of heparinises are produced by Flavobacterium heparinum and contains specific sequences of heparin.

Other Notes

Enzyme Commission Numbers: 4.2.2.7 Hep I and 4.2.2.8 Hep III

Packaging

Sold on the basis of Heparinase I units

Unit Definition

One unit will form 0.1 micromole of unsaturated uronic acid per hour at 7.5 at 25 degrees C using Heparin, Sodium as substrate for heparinase I.One unit will form 0.1 micromole of unsaturated uronic acid per hour at 7.5 at 25 degrees C using bovine kidney Heparan, Sulfate as substrate for heparinase III.One unit will form 0.1 µmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units. Package sizes are sold in Sigma units.