Monoamine Oxidase Activity Assay Kit, sufficient for 100 fluorometric?�tests

Not available outside of the UK & Ireland.

Application

Monoamine Oxidase Activity Assay Kit has been used to estimate MAO-A (monoamine oxidase A), MAO-B (monoamine oxidase B) and ALDH (aldehyde dehydrogenase) activities.

Features and Benefits

Compatible with high-throughput handling systems.

General description

Monoamine oxidases (MAO, EC 1.4.3.4) are a family of mitochondrial enzymes that catalyze the oxidative deamination of monoamines. In mammals, MAO consists of two isoenzymes, MAO-A and MAO-B. MAO dysfunction is thought to be responsible for a number of neurological disorders. Unusually high or low levels of MAO in the body have been associated with depression, schizophrenia, substance abuse, attention deficit disorder, migraines, and irregular sexual maturation. MAO inhibitors are one of the major classes of drug prescribed for the treatment of depression.

Principle

The Monoamine Oxidase Assay Kit provides a convenient fluorimetric means to measure MAO enzyme activity in biological samples. In the assay, MAO reacts with p-tyramine, a substrate for both MAO-A and MAO-B, resulting in the formation of H₂O₂, which is determined by a fluorimetric method (λ_ex = 530/λ_em = 585 nm). The assay is simple, sensitive, stable, and high-throughput adaptable. Unit definition: one unit of MAO catalyzes the formation of 1 µmole of H₂O₂ per minute under the assay conditions.

Suitability

Suitable for the determination of MAO activity in biological samples and for the screening of MAO inhibitors. Can determine either MAO-A, MAO-B or both MAO-A and MAO-B activity.