CETP Activity Assay Kit, Supplied by Roar Biomedical, Inc.

Not available outside of the UK & Ireland.

Analysis Note

Learn more about Roar Assay Kits for Studies in Lipoprotein Metabolism.

Application

Cholesteryl ester transfer protein (CETP) activity Assay Kit has been used for measurement of CETP activity in the plasma samples.

Features and Benefits

Compatible with high-throughput handling systems.

General description

Cholesteryl ester transfer protein (CETP) is present in normal human plasma. The protein transfers neutral lipids from high density lipoproteins (HDL) to very low density lipoprotein (VLDL) and low density lipoprotein (LDL). CETP plays an important role in lipoprotein metabolism and influences the reverse cholesterol transport pathway. The method is useful for measuring CETP activity in plasma or serum in all species that express CETP.

Principle

The CETP Activity Assay Kit uses a proprietary substrate that enables the detection of CETP-mediated transfer of neutral lipid from the substrate to a physiological acceptor. The transfer activity results in an increase in fluorescence intensity (λ_Ex=535 nm/λ_Em=587 nm). In a total volume of 200 µL, the assay is linear from 0.2 to 0.8 µL of normal human plasma. Assay results not affected by endogenous plasma HDL, LDL or VLDL concentrations.

Suitability

Suitable for the detection of cholesteryl ester transfer protein (CETP) in plasma / serum (fresh or frozen), recombinant or purified CETP.