Alcohol Dehydrogenase Activity Assay Kit, sufficient for 100 colorimetric?�tests

Not available outside of the UK & Ireland.

Application

Alcohol Dehydrogenase Activity Assay Kit has been used to determine the activity of alcohol dehydrogenase.

General description

Alcohol dehydrogenases (ADH) are a family of enzymes that catalyzes the conversion of alcohols to aldehydes, with the concomitant reduction of NAD⁺ to NADH. In humans, there are nine isozymes of ADH, with the majority of ADH activity occurring in the liver. ADH family members are the primary enzymes involved in alcohol detoxification. Genetic variations in ADH enzymes result in differences in ADH activity and tolerances for alcohol, and may regulate susceptibility to alcoholism.

Principle

The Alcohol Dehydrogenase Activity Assay kit provides a simple and direct procedure for measuring ADH activity in a variety of samples. ADH activity is determined using ethanol as the substrate in an enzyme reaction, which results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of ADH is the amount of enzyme that will generate 1.0 µmole of NADH per minute at pH 8.0 at 37 °C.

Suitability

Suitable for the detection of alcohol dehydrogenase in a variety of samples including tissue, cells and serum.