N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride, crystalline

Not available outside of the UK & Ireland.

Application

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used:for the immobilisation of trypsin onto self-assembled monolayers (SAMs)as a component for the preparation of collagen matricesfor the preparation of phosphoethanolamine(PEt)-conjugated sepharose

Biochem/physiol Actions

Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.

Packaging

1, 5 g in glass bottle