Proteasome Fraction II from rabbit, 5 mg/mL, Reticulocyte cell extract, solution

Not available outside of the UK & Ireland.

Application

Proteasome Fraction II is ideal for demonstrating ubiquitin- and ATP-dependent degradation/conjugation of radiolabeled or immunodetectable substrates. Isopeptidase inhibitor and a proteasome inhibitor are highly recommended for the accumulation of ubiquitin protein conjugates. Typical assay concentration for FII is 0.5 - 4 mg/ml.

Biochem/physiol Actions

Protein fraction of a cell extract that binds to anion-exchange resin.

Essentially ubiquitin and ATP free. Contains E1, most E2s, UCHs, and the proteasome 20S and 26S isoforms.

General description

Manufactured for Sigma by Boston Biochem., Inc.

Physical form

Solution in 25 mM HEPES, pH 7.6.