Not available outside of the UK & Ireland.
Application
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MRC1 in Human lymph node tissue lysate.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MRC1 in human lung tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MRC1 in RAW 264.7 cells.Flow Cytometry Analysis: 0.01 µg from a representative lot detected MRC1 in RAW 264.7 cells.Affinity Binding Assay: A representative lot of this antibody bound MRC1 peptide with a KD of 4.6 x 10-8 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-MRC1, clone 1C9 ZooMAb, Cat. No. ZRB1440, is a recombinant Rabbit monoclonal antibody that specifically targets MRC1 and is tested in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.
Immunogen
KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal region of human Macrophage mannose receptor 1 (MRC1).
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in lyophilized from PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
0.03 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Specificity
Clone 1C9 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Macrophage mannose receptor 1 (MRC1). It targets an epitope within 25 amino acids from the C-terminal region.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Target description
Macrophage mannose receptor 1 (UniProt: P22897; also known as MMR, C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like, Human mannose receptor, hMR, Macrophage mannose receptor 1-like protein 1, CD206, MRC1) is encoded by the MRC1 (also known as CLEC13D, CLEC13DL, MRC1L1) gene (Gene ID: 4360) in human. MRC1 is a single-pass type I membrane protein that mediates the endocytosis of glycoproteins by macrophages. It also functions in phagocytosis and plays a role in immune homeostasis by scavenging unwanted mannoglycoproteins. It is expressed on the surface of macrophages, immature dendritic cells and liver sinusoidal endothelial cells. It is a C-type lectin with eight C-type lectin domains and can bind both sulfated and non-sulfated polysaccharide chains. MRC1 is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form that contains a large extracellular domain (aa 19-1389), a transmembrane domain (aa 1390-13410), and a cytoplasmic tail (aa 1411-1456). The extracellular region includes an N-terminal cysteine-rich domain that binds glycoproteins bearing sulfated sugars that terminate in 4-SO4GalNAc, a fibronectin II (FNII) domain, and eight carbohydrate recognition domains that bind sugars like mannose and fucose with high affinity. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.