MONOCLONAL ANTI-HIF-1 ALPHA

Not available outside of the UK & Ireland.

Application

Monoclonal Anti-HIF-1 α antibody produced in mouse has been used in siRNA assay and western blot analysis.

Biochem/physiol Actions

Hypoxia-inducible factor-1(HIF1) plays an essential role in cellular and systemic homeostatic responses to hypoxia. During hypoxia, the two subunits of this factor undergo post-translational modifications which in turn promote transactivation. HIF-1α gene loss is associated with development of nonpapillary renal carcinomas. The encoded protein functions as a key regulator of the vascular endothelial growth factor (VEGF) under hypoxic response in human stromal cells in vitro. HIF-1α protein expression is often seen in invasive breast cancer. Experimental data demonstrates that downregulation of HIF-1α decreases tumorigenicity of MCF-7 (human breast adenocarcinoma cell line) cells and propose a promising combination of both anti-HIF-1 strategy and traditional chemotherapy to improve cancer treatment.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

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General description

Hypoxia-inducible factor-1 α (HIF1 α) gene encodes the α subunit of HIF-1. HIF-1α contains oxygen-dependent degradation (ODD) domain that mediates the interaction between HIF-1α and the E3 ubiquitin ligase complex, which in turn facilitates continuous poly ubiquitination of HIF-1α in normoxic cells. The gene encoding it is localized on human chromosome 14q23.2. HIF1 α belongs to the basic helix-loop-helix family of transcription factors.

Immunogen

Recombinant fragment corresponding to amino acids 329-530

Physical form

Solution in PBS, pH 7.4, 50% glycerol, and 0.09% sodium azide

Specificity

Specific for HIF1-alpha.