Monoclonal Anti-IgE antibody produced in mouse, clone 4H10, purified immunoglobulin, buffered aqueous solution

Not available outside of the UK & Ireland.

Application

The reagent is designed for Flow Cytometry analysis. Suggested working dilution for Flow Cytometry is 4 µg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Biochem/physiol Actions

Immunoglobulin E (IgE) binds very tightly to Fc receptors on basophils and mast cells.Thus, it plays a major role in stimulating allergic reactions. Elevated expression of the gene has been associated with the development of hyper IgE Syndrome (HIES), hay fever, atopic skin diseases such as eczema, anaphylactic shock and IgE-myeloma. In addition, overexpression of IgE is also observed in allergy/asthma.Therefore, increased levels of serum IgE can be considered as a diagnosis for asthma. IgE expression is down-regulated in congenital agammaglobulinemia and hypogammaglobulinemia because of faulty metabolism or synthesis of immunoglobulins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

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General description

The antibody 4H10 reacts with human IgE; it recognizes an epitope different from the ones recognized by BE5 and 4G7 antibodies to IgE.

Immunoglobulin E (IgE) is encoded by the gene mapped to human chromosome 14q32.33. IgE belongs to the immunoglobulin family and consists of epsilon(ε) heavy chain in the constant (C) region. IgE has a monomeric structure with an extra domain in the constant region.

Immunogen

Purified human IgE

Physical form

Solution in Tris buffered saline, pH 8.0, with 15 mM sodium azide.