Anti-PTPRF (C-terminal) antibody produced in rabbit; IgG fraction of antiserum

Not available outside of the UK & Ireland.

Application

Anti-PTPRF (C-terminal) antibody produced in rabbit has been used in an enzyme based assay of protein tyrosine phosphatase 1 (PTP1).

The antibody may be used in several immunochemical techniques including immunoblotting (~210, ~150, and ~85 kDa) and immunoprecipitation.

Biochem/physiol Actions

Protein tyrosine phosphatase, receptor type F (PTPRF) removes phosphate group from β-catenin, an event that may subsequently facilitate cell-cell adhesion and ensure the stability of the cadherin complex. This phosphatase has also been implicated in various cellular processes such as neurite growth, nerve regeneration, actin remodeling and regulation of insulin function. Insulin receptor, epidermal growth factor receptor (EGF) receptor, hepatocyte growth factor (HGF) receptor and rearranged during transfection (RET) have been reported as substrates of PTPRF.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Protein tyrosine phosphatase, receptor type F (PTPRF) consists of noncovalently bound subunits designated as E (extracellular) and P (phosphatase) subunits that are derived from a precursor protein by proteolytic processing. The E subunit (150 kDa) has three immunoglobulin-like domains and eight fibronectin III-like domains, and the P subunit (85 kDa) has a short extracellular domain, a transmembrane segment and tandem cytoplasmic PTP catalytic domains.

PTPRF or leukocyte common antigen-related protein (LAR) is a widely expressed protein tyrosine phosphatase with an extracellular receptor region that resembles a cell adhesion molecule.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

The antibody recognizes both the extracellular and the cytoplasmic subunits of human PTPRF. Anti-PTPRF (C-terminal) antibody is specific for the P- subunit and the whole PTPRF protein from Human origin. Detection of the PTPRF bands by immunoblotting is specifically inhibited by the immunizing peptide.