Anti-Pkm2 (Isoform M2). Antibody Produc

Not available outside of the UK & Ireland.

Application

Anti-PKM2 (isoform M2) antibody produced in rabbit has been used in:chromatin immunoprecipitation (chip) assaywestern blottingimmunofluorescenceimmunohistochemistry

Biochem/physiol Actions

Pyruvate Kinase (PK) is a key regulatory enzyme in glycolysis. Tumor cells have been reported to exclusively overexpress the embryonic M2 isoform. The tumor metabolome is characterized by high glycolytic turnover rate and tumor cells are able to proliferate under conditions of aerobic glycolysis, known as the Warburg effect. Knockdown of the M2 isoform in human cancer cell lines and its replacement by the M1 isoform has been shown to lead to reversal of the Warburg effect, and reduced ability to form tumors in mouse xenografts. Phosphorylation of the M2 isoform at Tyr105 inhibits its activity and is common in human cancers, suggesting that Tyr105 is a critical metabolic switch in cancer cells that promotes tumorigenesis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Pyruvate Kinase (PK) has four known isoforms, namely, L, R, M1 and M2. PK isoform M1 is expressed during the development of the embryo and is the dominant isoform in cardiac, brain and skeletal muscle tissues. The M2 isoform has been linked to cancer metastasis .

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Anti-PKM2 (isoform M2) antibody is specific for human PKM2 (isoform M2). The product is expected to react with rat and mouse PKM2. In immunoblotting, detection of the PKM2 (isoform M2) band is specifically inhibited by the PKM2 (isoform M2) immunizing peptide.