Anti-Raver1 (N-terminal) antibody produced in rabbit, approx. 1.0 mg/mL, affinity isolated antibody

Not available outside of the UK & Ireland.

Application

Anti-Raver1 (N-terminal) antibody produced in rabbit is suitable for: immunoblotting immunoprecipitation immunofluorescence

Biochem/physiol Actions

The protein ribonucleoprotein, PTB binding 1 (Raver1) is identified in two-hybrid screens by its ability to interact and colocalize with the cytoskeletal proteins α-actinin and vinculin. In skeletal muscle, the shuttling of Raver1 from nucleus to cytoplasm is associated with the differentiation of specific microfilament attachment sites. Based on an analysis of vinculin:Raver1 crystal structure, it was suggested that vinculin recruits Raver1 and its mRNAs cargo to focal adhesions, promoting restricted synthesis of adhesion complexes by the translational machinery.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The protein ribonucleoprotein, PTB binding 1 (Raver1), also known as ribonucleoprotein polypyrimidine tract-binding (PTB) protein 1, is a widely expressed multidomain protein. Raver1 is composed of three RNA recognition motifs (RRM) and of nuclear localization and nuclear export signals, allowing it to shuttle between the nucleus and the cytoplasm. The RAVER1 gene is mapped to human chromosome 19p13.2.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Anti-Raver1 (N-terminal) recognizes human Raver1.

Storage and Stability

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.