Anti-PORCN; clone 15G12.1

Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

ControlMouse brain tissue lysate

Application

Research CategorySignaling

Research Sub CategoryDevelopmental Signaling

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected PORCN in HeLa and A431 cells.

Anti-PORCN Antibody, clone 15G12.1 is an antibody against PORCN for use in WB & IC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PORCN is a multi-pass endoplasmic reticulum protein involved in the processing of wingless and other Wnt proteins that facilitate their secretion from Wnt-producing cells. Studies have shown mutations in the genes that encode for PORCN cause a pleiotropic X-linked dominant disorder, focal dermal hypolasia (FDH, also known as Goltz syndrome). Skeletal abnormalities such as syndactyly, ectordactyly, and brachydactyly, and some cases of osteopathia striata have also been attibuted to PORCN abnormalities.

Immunogen

Linear peptide corresponding to human PORCN.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified by Ion-Exchange Chromatography

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected PORCN in 10 µg of mouse brain tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~62 kDa observed.
Calculated molecular weight of 52 kDa The actual MW should be observed at ~45-65 kDa Helical membrane protein migration on SDS-PAGE may not exactly correlate with the calculated molecular weights (PNAS, 2009, 106:1760-1765).