Anti-STAM-binding protein; clone 4D12.2

Not available outside of the UK & Ireland.

Application

Research CategorySignaling

Immunocytochemistry analysis: 1:50 dilution from a representative lot detected STAM binding protein in A431 cells
DyLight^® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Research Sub CategoryUbiquitin & Ubiquitin Metabolism

This Anti-STAM-binding protein Antibody, clone 4D12.2 is validated for use in Western Blotting and Immunocytochemistry for the detection of STAM-binding protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

STAM-binding protein (STAMBP) is a critical metalloprotease in ubiquitin-mediated signaling events that specifically cleaves ′Lys-63′-linked polyubiquitin chains but does not cleave ′Lys-48′-linked polyubiquitin chains. STAM-binding protein is a deubiquitinating (DUB) isopeptidase also known as AMSH, for associated molecule with the SH3 domain of STAM. STAM-binding protein plays a role in cell growth and MYC induction, potentiates BMP signaling and is key in regulating the cell surface receptor-mediated endocytosis and ubiquitin-dependent sorting of receptors to lysosomes. STAM-binding protein interacts with other key proteins including STAM1, SMAD6/7, SMURF2, and CHMP3. STAM-binding protein is widely expressed and can be found localized to the nucleus, cytoplasm and endosome. Defects in STAM-binding protein result in a congenital syndrome named Microcephaly-capillary malformation syndrome.

Immunogen

GST-tagged recombinant protein corresponding to Human STAM-binding protein.

Legal Information

DyLight is a registered trademark of Pierce Biotechnology, Inc.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Routinely evaluated by western blotting on Jurkat cell lysate
Western Blotting Analysis: 1 µg/mL of this antibody detected STAM binding protein in 10 µg of Jurkat cell lysate

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~50 kDa observed