Anti-phospho-Cdk1(Thr14;Tyr15)cloneCP3.2

Not available outside of the UK & Ireland.

Analysis Note

ControlUV treated and untreated HeLa cell lysates

Application

Anti-phospho-Cdk1 (Thr14, Tyr15) Antibody, clone CP3.2 is a mouse monoclonal antibody for detection of phospho-Cdk1 (Thr14, Tyr15) also known as Cyclin-dependent kinase 1, or Cell division control protein 2 homolog & has been validated in WB, ICC.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cyclin dependent kinase 1 (Cdk1) regulates cell cycle progression. It is active as a heterodimeric complex with cyclins. Cdk1 associates with cyclin A at the end of S phase and targets proteins such as p53, BARD1, and BRCA2 involved in cell cycle progression, and others involved in DNA replication. At the end of the G2 phase, Cdk1 associates with cyclin B1 and B2 to phosphorylate multiple protein targets required for transition from G2 to M phase, and progression through M phase. Cdk1 itself undergoes phosphorylation at Thr14 and Tyr15 by PKMYT1, which prevents nuclear translocation of Cdk1. Cdk1 is also phosphorylated at Tyr15 by WEE1/2 which inhibits both Cdk1 activity and transition from G2 to M phase. Conversely, dephosphorylation of Thr14 and Tyr15 by CDC25A/B activates Cdk1 and triggers mitosis. Cdk1 may also regulate epigenetic gene silencing through phosphorylation of EZH2 and maintenance of histone H3 lysine 27 trimethylation, which has been implicated in cancer.

Immunogen

Linear peptide corresponding to human Cdk1 phosphorylated at Thr14 and phosphorylated at Tyr15.

Epitope: Phosphorylated Thr14 and phosphorylated Tyr15

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blot in UV treated and untreated HeLa cell lysates.

Western Blot Analysis: A 1:1,000 dilution from a representative lot detected phospho-Cdk1 (Thr14, Tyr15) in UV treated HeLa cell lysates.

Specificity

This antibody recognizes Cdk1 phosphorylated at Thr14 and phosphorylated at Tyr15.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~34 kDa observed. An uncharacterized band may be observed at ~62 kDa in some cell lysates.