Monoclonal Anti-Monocyte Chemotactic Protein-2 antibody produced in mouse, clone 35509.11, purified immunoglobulin, lyophilized powder

Not available outside of the UK & Ireland.

Application

Monoclonal Anti-Monocyte Chemotactic Protein-2 antibody may be used in ELISA at a concentration of 2.0 µg/ml. For immunoblotting a working concentration of 1-2 µg/ml may be used. The antibody is suitable for neutralization reactions; ND50 is 1-4 µg/ml.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monocyte Chemotactic Protein-2 (MCP-2) belongs to a family of C-C chemotactic cytokines. MCP-1, -3, -4, and -5 are the other five monocyte chemo attractants identified. The MCP cytokines are involved in recruitment of leukocytes to the sites of inflammation, and activate NK cells and CD4+ and CD8+ T lymphocytes. Additionally, MCP cytokines can activate basophils to secrete histamines and also attract eosinophils. MCPs play an important role in pathological conditions such as systemic sclerosis, myocardia and cardiac ischemia. Anti-monocyte chemotactic protein 2 antibody specifically reacts with human MCP-2. The antibody shows ﹤20% cross reactivity with recombinant human MCP-1 and recombinant mouse MIP-1α.

Immunogen

recombinant human MCP-2 expressed in E. coli.

Physical form

Lyophilized from a 0.2 µm filtered solution in phosphate buffered saline containing 5% trehalose

Preparation Note

Purified using protein A.

Specificity

Neutralizes the biological activity of recombinant human MCP-2. Shows ﹤20% cross-reactivity with recombinant human MCP-1 and recombinant mouse MIP-1α.