Anti-Human IgG (Fab specific), F(ab)2 fragment antibody produced in goat, affinity isolated antibody, buffered aqueous solution

Not available outside of the UK & Ireland.

Application

Anti-Human IgG (Fab specific), F(ab′)2 fragment antibody produced in goat has been used in enzyme linked immunosorbent assay (ELISA) and biosensor methodology.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Immunoglobulin G (IgG) is digested by papain and yields two Fab fragments, each of which can bind antigen, and a single Fc fragment. IgG is split by pepsin splits into an Fc fragment and a single dimeric F(ab)₂ that can cross-link as well as bind antigens.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulins (Igs) belongs to immunoglobulin super-family (IgSF). Igs in general, consist of two heavy (H) chains and two light (L) chains. The L chain can be either κ or a λ chain. Each chain contains one NH2-terminal “variable" (V) region and one or more COOH-terminal “constant” (C) regions. Each of which consists of two sandwiched β pleated sheets ′pinned′ together by a disulfide bridge. There are five main classes of Igs based on heavy chain C domains, they are, IgM, IgG, IgA, IgD, and IgE isotypes. IgG can be divided into four subclasses, IgG1, IgG2, IgG3, and IgG4. Each subclass possesses distinct biologic properties.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% sodium azide as preservative