Anti-HRD1/SYVN1 antibody produced in rabbit, approx. 1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Not available outside of the UK & Ireland.

Application

Anti-HRD1/SYVN1 antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Synoviolin (SYVN1) protects against endoplasmic reticulum (ER) stress-induced apoptosis through ER stress-associated protein degradation (ERAD). The really interesting new gene (RING) finger domain of SYVN1 mediates the transfer of ubiquitin from E2 to substrates and a proline-rich domain. E3 ubiquitin-protein ligase (HRD1) expression is strongly induced by ER stress. HRD1 interacts with parkin-associated endothelin receptor-like receptor (Pael-R), promoting its degradation, and protects neurons from cell death caused by the accumulation of Pael-R. HRD1 is also found to enhance the degradation and suppress the toxicity of polyglutamine-expanded huntingtin. In addition to its role in ERAD, HRD1 targets tumor suppressor gene p53 for proteasomal degradation. Overexpression of HRD1 is implicated in the pathogenesis of rheumatoid arthritis. The deletion of gene locus of SYVN1 is implicated in spastic paraplegia, optic atrophy, and neuropathy (SPOAN) disorder.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Synoviolin (SYVN1) is an endoplasmic reticulum (ER)-membrane resident E3 ubiquitin ligase. The SYVN1 gene is mapped to human chromosome 11q13.1. Also known as HRD1, this protein has five transmembrane domains and a really interesting new gene (RING) finger domain.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Specificity

Anti-HRD1/SYVN1 recognizes human and mouse HRD1/SYVN1.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.