Anti-APAF1 antibody produced in chicken, affinity isolated antibody, buffered aqueous solution

Not available outside of the UK & Ireland.

Application

Anti-APAF1 antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Biochem/physiol Actions

Apoptotic protease activating factor 1 is a human homolog of C. elegans CED-4 gene and is a key regulator gene of apoptosis. It acts as a central component of the intrinsic pathway of apoptosis. Loss of APAF-1 expression is involved with chemorefractory malignant melanoma and neuronal cell differentiation. Over expression of this gene is related to an undifferentiated state in the testicular germ cell tumor (TGCT) pathway and is involved in carcinogenesis of germ cell tumors. APAF-1, when bound to cytochrome c, may trigger the activation of caspase-3 leading to apoptosis.

Immunogen

Recombinant apoptotic protease activating factor isoform d

Immunogen Sequence: GI # 32483361, sequence 307-388

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.