Anti-GABAB Receptor 2 antibody produced in rabbit, affinity isolated antibody, lyophilized powder

Not available outside of the UK & Ireland.

Application

Anti-GABAB Receptor 2 antibody has been used in western blotting.

Anti-GABA_B Receptor 2 antibody produced in rabbit is suitable for western blotting at a working dilution of 1:200 using rat brain membranes.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)

Biochem/physiol Actions

The inhibitory neurotransmitter GABA signals through two distinct types of pre- and postsynaptic receptors, GABA_A and GABA_B. Both GABA receptors can regulate depression of synaptic transmission and be involved in the inhibition controlling neuronal excitability. The GABA transmitter interacts with three types of receptors: the ionotropic receptors GABA_A and GABA_C and the metabotropic GABA_B receptor (GABABR). GABABR is a G-protein coupled receptor that plays a key role in slow synaptic inhibition in the brain and spinal cord. GABABR is a heteromer with two subunits, GABABR1 and GABABR2. The amino acids within the second intracellular domain of GABA(B2) are essential for the coupling of GABA(B) receptor heterodimers to their downstream effector systems. The N-termini on both the GB1 and GB2 receptors are involved in the normal coupling of GABAB receptors to their physiological effectors, G(i) and G-protein-activated potassium channels (GIRKs).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The GABAB receptor (GABABR) is a heteromeric G-protein-coupled receptor. It has two subunits-GABAB1 (γ-aminobutyric acid type B receptor subunit 1) and GABAB2 (γ-aminobutyric acid type B receptor subunit 2). In the hippocampus, GABAB2 is present in dendrites and located in the extra-synaptic membrane of spines and dendritic shafts. This gene is located on rat chromosome 5q.

Immunogen

peptide CKDPIEDINSPEHIQRRLSL corresponding to residues 875-894 of human GABA_BR2.

Physical form

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide.

Reconstitution

Reconstitute the lyophilized vial with 50 µL or 200 µL deionized water, depending on package size. Further dilutions should be made using a carrier protein such as BSA (1-3%).

Target description

The GABA_BR2 was shown to be essential for trafficking and G-protein binding, while the GABA_B R1 subunit was demonstrated to be important for agonist and antagonist binding. GABA_BR2 is highly expressed in brain especially at the cerebral cortex, thalamus, hippocampus, and spinal cord and to a lesser extent in testis.