Anti-Destrin/ADF (AE-14) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

Not available outside of the UK & Ireland.

Application

Anti-Destrin/ADF antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:600 using whole extracts of human A-431 epidermoid carcinoma, rat PC-12 pheochromocytoma, and mouse NIH/3T3 fibroblasts, and a chemiluminescent detection reagent.

Biochem/physiol Actions

Destrin/ADF (Actin Depolymerizing Factor) is a small phosphoinositide-sensitive actin-binding protein capable of depolymerizing actin-filaments in vitro. It binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in a pH-dependent, Ca²⁺ independent manner. It intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. It is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, and cytokinesis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Destrin/ADF is usually found in regions containing dynamic actin pools such as the leading edge of migrating cells and neuronal growth cones and may also colocalize in cell nuclei. It is also present in ‘;Hirano bodies’; in certain brain neurons of dementia patients.

Destrin/ADF and cofilin are widely distributed in tissues of eukaryotes and both contain a nuclear localization sequence. It is found in various epithelial and endothelial cells but is practically absent from adult mouse heart and skeletal muscle cells. This gene is located on human chromosome 20p12.

Immunogen

synthetic peptide corresponding to amino acids 137-150 of human destrin/ADF.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.