Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8)

Not available outside of the UK & Ireland.

Application

Immunoblotting (1:2000)Immunofluorescence (1:200, indirect)Immunoprecipitation (see comments)

General description

Recognizes the ~95 kDa PSD95 protein.

This Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of PSD-95 (Ab-2).

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with HL-1 mouse myeloma cells (see application references). Recognizes the ~95 kDa PSD-95 protein.

Immunogen

recombinant, rat PSD95

Rat

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Brakeman, P.R., et al. 1997. Nature386, 284.Hualing, D., et al. 1997. Nature386, 279.Kennedy, M.B. 1997. Trends in Neurosci.20, 264.Sanes, J. 1997. Curr. Opin. Neurobiol.7, 93.Cho., K.-O., et al. 1992. Neuron9, 929.

Immunofluorescent staining of rat hippocampal cells shows a staining pattern coincident with NMDA receptor staining at synaptic sites consistent with PSD-95′s proposed role in receptor clustering. This antibody has also been reported to work for immunoprecipitation. Fixation with cold methanol is recommended for immunofluorescence. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µL in Glass bottle

Please refer to vial label for lot-specific concentration.

Physical form

In PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)