CYTOKER 20; MS X HU - 50 UG

Not available outside of the UK & Ireland.

Analysis Note

ControlPositive cell lines include HT-29, LoVo, DLD-1, SW 1116, CaCo-2 and RT-4.

Application

Research CategoryCell Structure

Detect Cytokeratin 20 using this Anti-Cytokeratin 20 Antibody, clone IT-Ks20.8 validated for use in WB, IH(P).

Research Sub CategoryCytokeratins

Western blotting
Immunohistochemistry: 1:10 dilution of stock on frozen tissues, with one hour incubation at room temp or overnight at 2°-8°C. For paraffin sections, 1:100 dilution of antibody with protease pretreatment and/or heat induced epitope retrieval recommended.
Cytological material

Studies of cultured cell lines HT-29, LoVo, DLD-1, SW1116, CaCo-2 & RT-4.

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IT-Ks 20.8 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.

Immunogen

Electrophoretically purified cytokeratin 20 from human intestinal mucosa

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: MABT150

Physical form

Protein A purified immunoglobulin lyophilized from PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide. Reconstitute with 1 mL distilled water.

Format: Purified

Specificity

The antibody is specific for the 46 kDa polypeptide (cytokeratin 20).

Storage and Stability

Once reconstituted, maintain at 2°-8°C for up to 12 months from date of receipt. For extended storage, aliquots can be stored at -20°C. Avoid repeated freeze/thaw cycles.