Anti-PCSK9

Not available outside of the UK & Ireland.

Application

This Anti-PCSK9 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of PCSK9.

Research Sub CategoryLipid SignalingLiver Physiology

Western Blotting Analysis: A representative lot of this antibody detected PCSK9 protein in treated HepG2 cell lysate (Jeong, H. J., et al. J. Lipid Res. 2008. 49: 399–409.)
Western Blotting Analysis: A representative lot of this antibody detected PCSK9 protein in siRNA transfected HepG2 cells without and with BBR treatment (Data compliments of Sahng Wook Park of Yongsei University College of Medicine).

Research CategorySignalingMetabolism

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PCSK9 protein is an important protein in the regulation of plasma cholesterol homeostasis. PCSK9 binds various lipid receptor protein members including low density lipoprotein receptors (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2) and promotes their degradation or sequestration. In neurons, PCSK9 regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways. PCSK9 is localized in cytoplasm but it is also secreted and thus can be found in endosomes, lysosomes, and endoplasmic reticulum and Golgi as well. PCSK9 is expressed in normal cells such as Schwann cells and pancreatic epithelial cells but PCSK9 is also expressed in many cancers and cancer cells lines as well.

Immunogen

His-tagged recombinant protein corresponding to Human PCSK9.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide with 30% glycerol.

Antigen Affinity Purified

Quality

Evaluated by Immunocytochemistry on HepG2 cells
Immunocytochemistry Analysis: 8.0 µg/mL of this antibody detected PCSK9 in HepG2 cells.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Target description

~75 kDa observed