APPLICATION (KPI - DOMAIN ); RB X-100 µL

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Western blot: 1:250 - 1:1,000

Immunoprecipitation: Requires reductions and alkylation of disulfide bonds.

Immunocytochemistry: On Cos7 and NteraZ cell lines, requires reduction and alkylation of disulphide bonds.

Immunohistochemistry: Works on formaldehyde fixed, paraffin embedded tissue, requires reduction and alkylation of disulphide bonds or microwaving.

Optimal working dilutions must be determined by end user.

Reduction /Alkylation protocol:

To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.

Anti-Amyloid Precursor Protein Antibody, KPI Domain is an antibody against Amyloid Precursor Protein for use in IP, WB, IC, IH.

Immunogen

APP.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Specificity

Amyloid Precursor Protein (APP), KPI Domain.