Anti-Monocarboxylate Transporter 1

Not available outside of the UK & Ireland.

Analysis Note

ControlRat brain membrane tissue lysate

Application

Immunohistochemistry Analysis: A 1:2,500 dilution from a representative lot detected rat skeletal muscle and rat renal cortex tissues.

Anti-Monocarboxylate Transporter 1 Antibody is a highly specific chicken polyclonal antibody, that targets MCT1 & has been tested in western blotting & IHC (Paraffin).

Research Sub CategorySignaling Neuroscience

Research CategoryNeuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monocarboxylate Transporter 1 (MCT1), also known as Solute carrier family 16 member 1 (SLC16A1), catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Monocarboxylate Transporter 1 is located in the cell membrane, and is widely expressed in both normal and cancer cells. Monocarboxylate Transporter 1 is involved in symptomatic deficiency in lactate transport (SDLT).

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of rat Monocarboxylate Transporter 1.

Linkage

Replaces: AB1286

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified chicken polyclonal IgY in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in rat brain membrane tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected rat brain membrane tissue lysate in 10 µg of rat brain membrane tissue lysate.

Specificity

This antibody recognizes the C-terminus of Monocarboxylate Transporter 1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

Bands are observed at ~44 kDa and ~55 kDa This protein has been observed at ~47 kDa (Sepponen, K., et al. (2003). Acta Physiol Scand. 177(1):79-86.). In addition this protein has been observed at ~55 kDa in certain cell lysates, except in the rumen where two distinct bands appear at ~47 kDa and ~55 kDa (Koho, N., et al. (2005). Comp Biochem Physiol A Mol Integr Physiol. 141(1):29-34.). It is also known to be glycosylated (Carpenter, L., et al. (1996). Biochim Biophys Acta. 1279(2):157-163).