Monoclonal Anti-AFX (FOXO4) antibody produced in mouse, clone AF3.10, purified immunoglobulin, buffered aqueous solution

Not available outside of the UK & Ireland.

Application

Monoclonal Anti-AFX (FOXO4) antibody can be used for western blot assays at 0.5-1µg/ml. The antibody is also suitable for use in indirect ELISA, immunocytochemistry and microarray applications.

Biochem/physiol Actions

Anti-AFX (FOXO4) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma AF3.10 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice. AFX plays a central role in the regulation of cell proliferation. It integrates signals from PI3K/PKB and Ras/Ral signaling pathways to regulate transcription of the cell cycle inhibitor p27kip1. AFX activation results in increased expression of p27kip1, resulting in cell cycle arrest. Overexpression of AFX blocks cells cycle progression at G1 by a p27kip1 dependent mechanism.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Forkhead box O4 (FOXO4) belongs to the FOXO transcription factor family that regulates growth, cell cycle, metabolism, stress tolerance, aging and apoptosis in cells. Studies have reported that USP7 modulates FOXO4 transcription. Moreover, it has been demonstrated that peroxide stress acetylates FOXO4, whereas the longevity protein hSir2^SIRT1 deacetylates it.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Monoclonal Anti-AFX (FOXO4) recognizes human AFX.