Anti-ATG4B antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

Not available outside of the UK & Ireland.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Immunohistochemistry (1 paper)Western Blotting (1 paper)

Anti-ATG4B antibody produced in rabbit has been used in western blottingimmunohistochemistry

Biochem/physiol Actions

ATG4B (autophagy-related 4B cysteine peptidase) participates in the modulation of autophagy in mammalian cells. It is also involved in light chain 3 (LC3) lipidation. ATG4B is responsible for the processing of the mammalian Atg8 homologues: Golgi-associated ATPase enhancer of 16 kDa (GATE16), gamma-aminobutyric-acid-type-A-receptor-associated protein (GABARAP), and LC3. It removes the carboxyl-terminal region of proLC3 immediately after its synthesis, generating the soluble LC3-I and exposing a carboxyl-terminal Gly.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ATG4B is a member of the C-54 family of cysteine proteases that is involved in cell degradation through autophagy.

ATG4B (autophagy-related 4B cysteine peptidase), a mammalian orthologue of yeast Atg4, is a cysteine protease. It is widely expressed in human and rat tissues, being most abundant in the brain. This gene is mapped to human chromosome 2q37.

Immunogen

synthetic peptide corresponding to amino acids 6-22 of human ATG4B, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in mouse.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.