Anticorps Anti - ATG5 (N-terminal) produit en lapin, anticorps isolé affinité, solution PBS

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Rabbit anti-ATG5 has been used for immunofluorescence and chemiluminescent western blot analyses.

Rabbit anti-ATG5 (N-terminal specific) was used for western blot analysis to detect the level of ATG5 in SK-N-MC cells after transduction with an adenovirus expressing a shRNA specific to ATG5.

Anti-ATG5 (N-terminal) antibody has been used:in immunoblottingin immunofluorescencein western blotting

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)

Biochem/physiol Actions

Atg5 (Apg5) is a 32 kDa protein that is required for autophagy. Atg5 is covalently modified by Atg12, a ubiquitin-like modifier. The Atg12-Atg5-Atg16 complex thus formed, localizes to autophagosome precursors and regulates autophagosome formation. Studies have reported that Atg5 fragment that is formed by calpain cleavage has pro-apoptotic properties.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

synthetic peptide corresponding to amino acids 2-15 of human ATG5, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Specificity

Rabbit anti-ATG5 (N-terminal) recognizes human, rat, and mouse Atg5-Atg12 complex.