Anti-CD28 Mouse mAb (ANC28.1/5D10)

Not available outside of the UK & Ireland.

Application

Flow Cytometry (5 µg/ml)r>Frozen Sections (see comments)r>Immunoprecipitation (see comments)r>T cell Co-stimulation (see comments)

General description

Recognizes CD28 on human T cells and BHK cells transfected with human CD28.

Protein A purified mouse monoclonal antibody. Recognizes the CD28 protein.

Anti-CD28, mouse monoclonal, clone ANC28.1/5D10, recognizes CD28 on human T cells & human CD28 transfected BHK cells. It is validated for FC, IP, IHC on frozen sections, and T cell co-stimulation.

Immunogen

human CD28 Ig fusion protein and HPB-MLT human tumor cells

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kariv, I., et al. 1996. J. Immunol.157, 29.61st Forum in Immunology. 1995. Res. Immunol.146, 127.Jenkins, M.K., and Johnson, J.G. 1993. Curr. Opin. Immunol.5, 361.

Monomeric and cross-linked antibody will stimulate expression of IL-2 from CD28+ cells. FACS was performed on 5x10⁵ cultured HPB-MLT cells using anti-CD28 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Reconstitution

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Warning

Toxicity: Standard Handling (A)