Veratox® for Peanut Allergen is used for the quantitative analysis of peanut residues in food products such as cookies, crackers, chocolate bars, ice cream and cereals. Can also be used to screen environmental swabs.
The Veratox for Peanut Allergen test is a sandwich enzyme-linked immunosorbent assay (S-ELISA). Peanut protein residue is extracted from samples with a phosphate buffered salt solution (PBS) by shaking in a heated water bath, followed by centrifugation or filtration. Extracted peanut residue is sampled and added to antibody-coated wells (capture antibody) where it binds to the antibody during an incubation. Any unbound peanut residue is washed away and a second antibody (detector antibody), which is enzyme labelled, is added. The detector antibody binds to the already bound peanut residue. After a second wash, substrate is added. Colour develops as a result of the presence of bound detector antibody. Red Stop reagent is added and the colour of the resulting solution is observed. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form a standard curve, and the sample optical densities are plotted against the curve to calculate the exact concentration of peanut protein.
- Accurate and easy-to-use
- Quantitate or screen samples
- High specificity
- Analyte: peanut
- Platform: ELISA
- Result Type: quantitative
- Approvals: AOAC PTM #030403
- Limit of Detection: 1 ppm peanut
- Time to Result: 30 minutes
- Quantity per Package: 48 tests
This product has met the following criteria to qualify for the following awards: