HISTRAP EXCEL 1X1ML

Code: GE29-0485-86 D2-232

Features and Benefits

• HiTrap columns prepacked with Ni Sepharose excel medium, resistant to chelating and reducing agents • Load eukaryotic cell culture samples containing ...


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Features and Benefits

• HiTrap columns prepacked with Ni Sepharose excel medium, resistant to chelating and reducing agents • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity • Increase target protein yield and decrease degradation through reduced and simplified sample handling • HisTrap excel columns allow direct purification of cell-free, unclarified samples.

General description

HisTrap excel columns are prepacked with Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted by immobilized metal ion affinity chromatography (IMAC). The media is resistant to chelating and reducing agents making the medium an excellent choice for the purification of excreted proteins.

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

HisTrap is a trademark of GE Healthcare

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

working range2 - 13
average diameter90 µm
parameter1 ml/min - 4 ml/min flow rate (H2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 1 ml/min is recommended.)
parameter5 bar
capacity≥10 mg binding capacity (histidine-tagged protein)(H2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 1 ml/min is recommended.)
capacity≥10 mg binding capacity (histidine-tagged protein/ml sedimented medium)(Dynamic binding capacity was tested with 0.5 mg/ml (histidine)6 tagged pure protein (Mr 43 000) in EX-CELL® 420 Insect serum-free medium (capacity at 10% breakthrough) or (histidine)6-tagged protein (Mr 28 000). Column volume was 1 ml and flow rate 1 ml/min. Binding capacity is sample-dependent.)
bed volume1 mL
column I.D.7 mm
mfr. no.GE Healthcare, 29-0485-86
cleaning2 - 14 (Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.)
shelf lifePlease be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
bed size7 mm × 25 mm
packagingpack of 1 mL
availabilitynot available in North America
matrixhighly cross-linked 6% agarose
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