High Sensitivity Pyrophosphate Assay Kit, sufficient for 200 fluorometric�tests

Not available outside of the UK & Ireland.

General description

Pyrophosphate (PP_i) is produced by a number of biochemical reactions such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation, and the formation of fatty acid-coenzyme A esters.

Principle

The pyrophosphate concentration of a sample is determined by the use of a unique fluorogenic pyrophosphate sensor in which the presence of pyrophosphate results in the production of a fluorescent product (λ_ex =370/λ_em = 470 nm) proportional to the pyrophosphate present. This assay is simpler and more robust than traditional enzyme-based methods and is ideal for screening enzyme activity or enzyme inhibitors.

Suitability

The Pyrophosphate Assay Kit provides a simple and direct flourescent microplate procedure for measuring pyrophosphate in a variety of samples.