3-Hydroxy-3-methylglutaryl-CoA reductase human, solution

Not available outside of the UK & Ireland.

Application

3-Hydroxy-3-methylglutaryl-CoA reductase human has been used:in anti-HMGCR enzyme linked immunosorbent assay (ELISA)as an antigen to measure anti-HMGCR antibodies by blot analysisin HMG-CoA reductase activity assaysas an antigen to compare in-house antigen via sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)/western blot

Biochem/physiol Actions

Hydroxy-3-methylglutaryl-CoA reductase catalyzes the committed step in cholesterol biosynthesis.

3-Hydroxy-3-methylglutaryl-CoA reductase/HMGCR serves as a rate limiting enzyme in mevalonate pathway. It also controls the oncogenic Hippo transducers TAZ/YAP. HMGCR also perform as a genetic modifier for Alzheimer′s disease (AD).

Physical form

Supplied as a solution containing 250 µg protein in 50 mM Tris pH 7.5, with 5 mM DTT, 1:200 Protease Inhibitor Cocktail (Catalog Number P8340) and 50% (w/v) glycerol.

Physical properties

Consists of the HMG CoA reductase catalytic domain fused to GST protein.

Unit Definition

One unit will convert 1.0 µmole of NADPH to NADP⁺ per minute at 37 °C.