ECL Select Western Blotting Detection Reagent, Cytiva, RPN2235

Analysis Note

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Application

Western Blotting

Components

includesSolution A (Luminol solution, 50 mL)Solution B (Peroxide solution, 50 mL)sufficient for 1000 cm² membranecomplete packsizefor 1000 cm² membrane

Features and Benefits

• Improved sensitivity: Produces a very high signal intensity• Quantitative measurements: Linear dynamic range allowing for quantitation of medium to very low abundant proteins• Works with a wide range of antibody dilutions, reducing the need for lengthy optimization• Optimized for imaging with ImageQuant LAS 4000 (CCD-based imaging) and compatible with Amersham^™ Hyperfilm• Compatible with Rainbow^™ Molecular Weight Markers and Amersham^™ ECL^™ DualVue Western Blotting Markers.• Optimized for use with Amersham^™ Hybond-P (PVDF) membranes and compatible with Amersham^™ Hybond^® ECL^™ (nitrocellulose) membranes

General description

Amersham^™ ECL^™ Select: Western blotting detection reagent with highly intense signal output, for detection of medium to very low protein levels.Amersham^™ ECL^™ Select is a high sensitivity reagent for chemiluminescent Western blotting detection. High signal intensity makes Amersham^™ ECL^™ Select suitable for the most demanding Western blotting applications, allowing for detection of minute protein quantities. Its long lasting signals allow for multiple exposures and a convenient time window between experiment and analysis. Signals can be detected with CCD camera or X-ray film.Amersham^™ ECL^™ Select typically exhibits a dynamic range that is complementary to Amersham^™ ECL^™ Prime, and similar to Amersham^™ ECL^™ Advance.

Legal Information

Rainbow is a trademark of GE Healthcare

Hybond is a registered trademark of GE Healthcare Companies

ECL is a trademark of GE Healthcare

Amersham is a trademark of GE Healthcare