HSAVEC-C

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Primary Human Saphenous Vein Endothelial Cells (HSaVEC) are isolated from human saphenous veins from single donors and are provided in a cryopreserved format. The saphenous vein is often used for autotransplantation in coronary artery bypass operations, when arterial grafts are not available or many grafts are required.

Cell Line Origin

Vein

Other Notes

Recommended Plating Density: 5000 - 10000 cells per cm²Passage After Thawing: P2Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15

Quality

Rigid quality control tests are performedfor each lot of Endothelial Cells fromlarge vessels. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. von Willebrand Factor (vWF) and CD31, as well as Dil-Ac-LDL uptake assays are also carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Subculture Routine

Click here for more information.

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.