DAPI Stain

Not available outside of the UK & Ireland.

Biochem/physiol Actions

Reversible: yes

Primary TargetDNA

Cell permeable: yes

General description

Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.

Cells are typically stained at 1 µg/ml.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Mathieu, C., et al. 1995. Exp. Cell Res.216, 13.Cubria, C., et al. 1994. Comp. Biochem. Physiol. C.105, 251.Hotz, M.A., et al. 1994. Cytometry15, 237.Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods26, 249.Miller, T., et al. 1993. Biotechniques15, 1042.r>Gray, J.W., et al. 1982. Science238, 323.Hessling, J.J., et al. 1980. J. Immunol. Methods38, 315.r>Russels, W.C., et al. 1975. Nature253, 461.

Packaging

Packaged under inert gas

Physical form

A 5 mg/ml (14.27 mM) sterile-filtered solution of DAPI, 2HCl (Cat. No. 268298) in H₂O.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.

Warning

Toxicity: Standard Handling (A)