5(6)-Carboxyfluorescein, BioReagent, suitable for fluorescence, >=95% (HPLC)

Not available outside of the UK & Ireland.

Application

5(6)-Carboxyfluorescein is used to characterize tumor cells and normal cells in vivo. It labels proteins derived through N-hydroxysuccinimide (NHS) ester-mediated derivatization. (4) 5(6)-Carboxyfluorescein is suitable for detecting cell viability while developing an in vitro anthelmintic drug assay.

General description

5(6)-Carboxyfluorescein, also known as 5(6)-FAM, is a fluorescent tracer and available as a mixture of 5-Carboxyfluorescein and 6-Carboxyfluorescein isomers. The fluorescence of 5(6)-Carboxyfluorescein is determined by the xanthene moiety. 5(6)-Carboxyfluorescein has two characteristic properties: It has 2 wavelengths for maximum absorbance (465 and 490nm). And its fluorescence emission, at 515nm(max.), increases as a function of pH in the physiological range of 6-7.4.

Other Notes

A probe of intracellular pH