TransPlex Whole Transcriptome Amplification Kit, DNA polymerase separate.

Not available outside of the UK & Ireland.

Application

Suitable for use with downstream applications including: qPCR microarray analysis cloning

TransPlex^® Whole Transcriptome Amplification Kit has been used to synthesize double-stranded cDNA. It has also been used in the amplification of cDNA.

Features and Benefits

Amplification of total RNA in less than 4 hours with less than 30 minutes of "hands-on" time Only 5 ng of starting material required to produce a highly representative library from total RNA Microgram quantities of amplification product generated from intact RNA from tissue, cultured cells, serum, or degraded RNA from formalin-fixed paraffin-embedded samples

General description

TransPlex^®, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3′-bias. Microgram quantities of amplification product generated from tissue, cultured cells, formalin-fixed samples, or serum are suitable for downstream applications such as qPCR and microarray analyses.

Legal Information

TransPlex is a registered trademark of Rubicon Genomics, Inc.

Principle

The WTA process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. As polymerization proceeds, displaced single strands serve as new templates for primer annealing and extension. The resultant OmniPlex cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence, is then amplified by PCR with the universal primer to produce WTA product.