A modification of Christensen's medium by Maslen this medium is generally used to detect rapid urease activity of Proteus spp. although it can be used to detect urease activity of other enterobacteriaceae including urease producing Salmonella spp. and Shigella spp. Unlike Christensen's medium when used for the later purpose it is not necessary to increase the incubation time.
The peptone provides the required nitrogen, carbon and vitamins. Glucose is a fermentable carbohydrate. The disodium phosphate and potassium dihydrogen phosphate are buffers and sodium chloride maintains osmotic balance. Phenol red is a pH indicator.
- Storage temperature: 10 to 30°C
- Dehydrated medium appearance: fine powder
- Prepared medium appearance: liquid
- Dehydrated medium colour: beige
- Prepared medium colour: pale orange
- pH: 6.8 ± 0.2
- Recommended incubation: aerobically at 37°C ± 1°C for up to 4 to 6 hours
Ce produit répond aux critères suivants pour être admissible aux récompenses suivantes :