Malt extract agar (pH 3.5) is a general-purpose medium for the detection, isolation and enumeration of yeasts and moulds. Malt extract agar is recommended for the detection and isolation and enumeration of yeasts and moulds. The medium is also used in the testing of foods and various materials and for the cultivation of the strains for microbiological vitamin assays. Malt extract agar has been used to cultivate fungi and yeast cultures in the sugar industry, in the manufacturing of syrups, soft drinks, and foods.
Malt extract and mycological peptone supply the carbon, nitrogen and nutrient sources required by the organisms. The acidic pH of this medium allows for optimal growth of moulds and yeasts while restricting bacterial growth. Malt extract agar is used for the cultivation of fungi although, with the prolonged incubation times needed, cultures may become overgrown with bacteria; for this reason, the pH of the medium is lowered to 3.5 by the addition of lactic acid, which increases the selectivity of the medium.
- Storage temperature: 2 to 8°C
- Appearance: firm gel
- Colour: pale brown
- pH: 3.5 ± 0.2
- Plate size: 90mm
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