3,3-Diaminobenzidine tetrahydrochloride hydrate, ISOPAC

Not available outside of the UK & Ireland.

Application

3,3′-Diaminobenzidine tetrahydrochloride hydrate has been used in immunohistological staining procedure and in situ hybridization.

Biochem/physiol Actions

DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown end-product for use in immunohistological and immunoblotting staining procedures.

Caution

Injecting a compatible solvent permits preparation of any desired strength solution without exposure.

Legal Information

Isopac is a registered trademark of Sigma-Aldrich Co. LLC

Packaging

Weight approximate. Packaged in a 100 mL serum bottle with silicon/PTFE septum and aluminum tear seal.

100 mg in serum bottle

Reconstitution

Dissolving the contents in 100 mL of solvent yields a 0.1% solution.