The LightBench® Discover is a versatile 3-in-1 instrument delivering automated gel electrophoresis for DNA Size Selection, Large Fragment Analytics (>150 kb) and Fluorometric Quantification applications, all in one compact footprint.
The instrument offers a fast, cost-effective solution for research laboratories to streamline workflows and reduce setup and servicing costs, whilst delivering precise and accurate target enrichment as well as high-accuracy analytics.
Powered by Ranger® Technology, it uses pulsed field inversion technology to resolve large DNA, allowing fragment length analysis of DNA up to 150 kb in less than 2 hours.
The inbuilt camera enables real time imaging allowing the electrophoresis progress to be monitored live and parameters to be adjusted mid run without losing precious sample.
The LightBench® Discover Ranger Analytics software provides mass-based and molarity-based quality control metrics, including average fragment size, sample purity and sample quality, to ensure accurate analysis and reliable results. The new Sample Quality Number (SQN) is used to assess DNA quality.
The software has two pre-loaded software protocols for large fragment analysis which enable quick and easy set-up. The 3 to 75 kb protocol is ideal for PacBio users and the 20 to 150 kb protocol is ideal for resolving very large DNA.
To compliment the LightBench® Discover there are a range of consumables (sold separately). These kits are comprised of a 0.5% agarose gel cassette, which facilitates larger DNA fragments to move through the gel and enables 20 samples to be analysed in one run. The Dual Dye Loading Buffer (DDLB) has an orange dye to aid usability, and the robust large fragment analytics DNA ladder is comprised of DNA bands ranging from 3 to 50 kb. These kits have a 12-month shelf life from date of manufacture.
The LightBench® Discover can also perform a plate-based fluorometric assay to accurately determine the concentration of DNA samples. This quantification data integrates with sizing data from fragment length analysis, and is used, for example, to ensure optimum molar dilution of samples prior to sequencing.
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