Protein G High Performance Spintrap, Cytiva, 28-9031-34, pack of 16 columns

Code: ge28-9031-34 D2-232

Analysis Note

www.gelifesciences.com.

Application

Protein G Sepharose® HP has high affinity for the Fc region of IgG from a varie...


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$293.33 PK16
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Analysis Note

www.gelifesciences.com.

Application

Protein G Sepharose® HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen to be eluted separately (cross-link protocol) or together with the antibody (classic protocol).There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

Features and Benefits

• For simple, small-scale purification of antibodies or enrichment of specific Proteins in prepacked spin columns that are ready to use.• Classic and cross-link protocols provide flexibility.• Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.• Fast binding kinetics and high capacity provides high yield.• Prepacked with Protein G Sepharose® High Performance for coupling of antibodies of IgG subclasses.• Easy scale-up with HiTrap® Protein G HP prepacked columns.

General description

protein G HP SpinTrap™ is a prepacked, single-use spin column containing protein G Sepharose® High Performance.

Legal Information

HiTrap is a registered trademark of GE Healthcare

SpinTrap is a trademark of GE Healthcare

Sepharose is a registered trademark of GE Healthcare

avg. part. size34 µm
capacity﹥1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
capacity﹥1 mg binding capacity(human IgG/column)
mfr. no.GE Healthcare, 28-9031-34
packagingpack of 16 columns
working range3 - 9 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
cleaning2 - 9 (pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
storage temp.2-8°C
shelf lifePlease be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
featurewetted part: no
matrixhighly cross-linked 6% agarose
This product has met the following criteria to qualify for the following awards:



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