D-lactique déhydrogénase de Lactobacillus leichmanii, poudre lyophilisée, 150-300 unités/mg de protéines

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

In the food industry, the primary catalysis is coupled to conversion of NADH and H⁺ to NAD⁺ with diaphorase coupled with converting the non-fluorescent resazurin to the highly fluorescent substance resorufin to measure the content of D-lactate in food products.

Biochem/physiol Actions

D-lactic dehydrogenase catalyzes the conversion of pyruvate into D-lactate, with oxidation of NADH to NAD⁺. D-lactic dehydrogenase can also catalyze the reverse reaction, conversion of D-lactate into pyruvate with reduction of NAD⁺ to NADH.

Packaging

500, 2500 units in poly bottle

Physical form

Lyophilized powder containing phosphate buffer salts

Unit Definition

D-lactic dehydrogenase catalyzes the conversion of pyruvate into D-lactate, with oxidation of NADH to NAD⁺. D-lactic dehydrogenase can also catalyze the reverse reaction, conversion of D-lactate into pyruvate with reduction of NAD⁺ to NADH.

One unit will reduce 1.0 µmole of pyruvate to D-lactate per min at pH 7.0 at 25 °C.