Non disponible en dehors du Royaume-Uni et de l'Irlande
Application
L-Glutamate Oxidase from Streptomyces sp may be used:as a reference enzyme in hydrogelpoly(carbamoylsulphonate) based immobilization for biosensor developmentas a component of pencil graphite electrode for biosensing L-glutamate
Biochem/physiol Actions
L-Glutamate Oxidase (LGOX) catalyzes the oxidative deamination of L-glutamate to α-ketoglutarate, ammonia and hydrogen peroxide. LGOX is industrially produced and is exploited majorly as a biosensor for L-glutamate detection and in the L-glutamate kits.
General description
L-Glutamate Oxidase (LGOX) from Streptomyces sp is secreted extracellularly in the wheat bran pre cultivation medium. It corresponds to a molecular weight of 120 kDa with monomer subunit of 61 kDa. The structure of LGOX comprises of hexamer and has flavin adenine dinucleotide prosthetic group.
This product has a 6xHistidine tag with a thrombin cleavage site.
Unit Definition
One unit will form 1.0 micromole of alpha-ketoglutaric acid from L-glutamic acid per minute at pH 7.4 at 30 deg C.
Ce produit répond aux critères suivants pour être admissible aux récompenses suivantes :